open access

Vol 63, No 5 (2013)
Research paper (original)
Published online: 2013-10-25
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Identification and significance of circulating endothelial progenitor cells in patients with hepatocellular carcinoma

Włodzimierz Otto, Maria Król, Maciej Maciaszczyk, Janusz Sierdziński, Bogusław Najnigier, Marek Krawczyk
DOI: 10.5603/NJO.2013.0035
·
Nowotwory. Journal of Oncology 2013;63(5):383-394.

open access

Vol 63, No 5 (2013)
Original article
Published online: 2013-10-25

Abstract

Aim of study. The study identified and assessed the role of endothelial progenitor cells in the peripheral blood circulation of patients with hepatocellular carcinoma.

Material and methods. There were 133 HCC patients who qualified for liver resection, 45 for liver transplantation, and 42 for palliation. Control groups consisted of 43 healthy and 42 cirrhotic individuals. Circulating HSCs and EPCs were measured by phenotypic analysis of fresh blood samples in a flow cytometer according to the approval of ISHAGE. Endothelial progenitor cells (EPCs) were defined and enumerated as CD34(+),133(+),CD45(dim), CD309(+). Results were evaluated statistically indicating the rate of endothelial progenitor cells (%EPCs) in the fraction of hematopoietic stem cells (HSCs — defined as CD34(+),CD133(+),CD45(dim), the subpopulation of leukocytes (WBC) — defined asCD45(+) cells.

Results. Significant differences were found in the rate of the EPC fraction and in the HSC subpopulation between cancer patients and healthy individuals (the test used was U-Mann-Whitney: Chisq = –5.92, p < 0.0001 and Chisq = 3.85,p < 0.0001, respectively), and between patients with cirrhosis and with a healthy liver (Chisq = –6.09, p < 0.0001 and Chisq = 2.47, p < 0.01, respectively). The differences between patients with HCC and with liver cirrhosis were statisticallynot significant.

Conclusions. Increasing the rate of hematopoietic stem cells and endothelial progenitor cells in peripheral blood circulation indicates the importance for HCC development.

Abstract

Aim of study. The study identified and assessed the role of endothelial progenitor cells in the peripheral blood circulation of patients with hepatocellular carcinoma.

Material and methods. There were 133 HCC patients who qualified for liver resection, 45 for liver transplantation, and 42 for palliation. Control groups consisted of 43 healthy and 42 cirrhotic individuals. Circulating HSCs and EPCs were measured by phenotypic analysis of fresh blood samples in a flow cytometer according to the approval of ISHAGE. Endothelial progenitor cells (EPCs) were defined and enumerated as CD34(+),133(+),CD45(dim), CD309(+). Results were evaluated statistically indicating the rate of endothelial progenitor cells (%EPCs) in the fraction of hematopoietic stem cells (HSCs — defined as CD34(+),CD133(+),CD45(dim), the subpopulation of leukocytes (WBC) — defined asCD45(+) cells.

Results. Significant differences were found in the rate of the EPC fraction and in the HSC subpopulation between cancer patients and healthy individuals (the test used was U-Mann-Whitney: Chisq = –5.92, p < 0.0001 and Chisq = 3.85,p < 0.0001, respectively), and between patients with cirrhosis and with a healthy liver (Chisq = –6.09, p < 0.0001 and Chisq = 2.47, p < 0.01, respectively). The differences between patients with HCC and with liver cirrhosis were statisticallynot significant.

Conclusions. Increasing the rate of hematopoietic stem cells and endothelial progenitor cells in peripheral blood circulation indicates the importance for HCC development.

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About this article
Title

Identification and significance of circulating endothelial progenitor cells in patients with hepatocellular carcinoma

Journal

Nowotwory. Journal of Oncology

Issue

Vol 63, No 5 (2013)

Article type

Research paper (original)

Pages

383-394

Published online

2013-10-25

Page views

1065

Article views/downloads

2859

DOI

10.5603/NJO.2013.0035

Bibliographic record

Nowotwory. Journal of Oncology 2013;63(5):383-394.

Authors

Włodzimierz Otto
Maria Król
Maciej Maciaszczyk
Janusz Sierdziński
Bogusław Najnigier
Marek Krawczyk

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