Vol 48, No 3 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2010-11-13
Assessment of S100 protein expression in the epididymis of juvenile and adult European bison.
Elżbieta Czykier, Maciej Zabel, Joanna Surdyk-Zasada, Agnieszka Lebelt, Beata Klim
DOI: 10.2478/v10042-10-0020-4
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Folia Histochem Cytobiol 2010;48(3):333-338.
Vol 48, No 3 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2010-11-13
Abstract
In our study, we decided to compare S100 protein expression in the material obtained from the epididymes of 5- and 12-month-old calves, and adult European bison, and to detect any differences in S100 expression according to the animal age and size of the organ examined. We used the epididymes obtained from 6 adult European bison aged 6-12 years, from 6 at the age of 12 months and 6 calves aged 5 months. Immunocytochemical reactions were performed using the avidin-biotinylated-peroxidase (ABC) technique according to HSU. Specific polyclonal rabbit antiserum against bovine S100 protein (Bio Genex Laboratories) at a dilution at 1:400 was applied. We found the expression of S100 protein in endothelial cells of arteries, veins and lymphatic vessels in all the study animals. At the same time, we found no differences in the expression of S100 protein in vascular endothelial cells. Our observations seem to indicate that S100 expression in endothelial cells of European bison epididymis is not correlated with age or maturity of the organ tested. We found S100 protein in smooth muscle cells of arteries and veins in all European bison specimens examined. Interestingly in the current study, in young 5-month-old sexually immature European bison specimens we observed weaker expression of S100 protein in smooth muscle cells of small vessels as compared to the same cell type both in large vessels in these animals and in small vessels in adult specimens.
Abstract
In our study, we decided to compare S100 protein expression in the material obtained from the epididymes of 5- and 12-month-old calves, and adult European bison, and to detect any differences in S100 expression according to the animal age and size of the organ examined. We used the epididymes obtained from 6 adult European bison aged 6-12 years, from 6 at the age of 12 months and 6 calves aged 5 months. Immunocytochemical reactions were performed using the avidin-biotinylated-peroxidase (ABC) technique according to HSU. Specific polyclonal rabbit antiserum against bovine S100 protein (Bio Genex Laboratories) at a dilution at 1:400 was applied. We found the expression of S100 protein in endothelial cells of arteries, veins and lymphatic vessels in all the study animals. At the same time, we found no differences in the expression of S100 protein in vascular endothelial cells. Our observations seem to indicate that S100 expression in endothelial cells of European bison epididymis is not correlated with age or maturity of the organ tested. We found S100 protein in smooth muscle cells of arteries and veins in all European bison specimens examined. Interestingly in the current study, in young 5-month-old sexually immature European bison specimens we observed weaker expression of S100 protein in smooth muscle cells of small vessels as compared to the same cell type both in large vessels in these animals and in small vessels in adult specimens.
Title
Assessment of S100 protein expression in the epididymis of juvenile and adult European bison.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 48, No 3 (2010)
Article type
Original paper
Pages
333-338
Published online
2010-11-13
Page views
2016
Article views/downloads
1655
DOI
10.2478/v10042-10-0020-4
Bibliographic record
Folia Histochem Cytobiol 2010;48(3):333-338.
Authors
Elżbieta Czykier
Maciej Zabel
Joanna Surdyk-Zasada
Agnieszka Lebelt
Beata Klim
About this article