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Vol 49, No 2 (2011)
Brief communication
Submitted: 2011-12-19
Published online: 2011-07-11
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Inflammatory bowel disease — is there something new in the immunological background?

Andrzej Marszałek, Ryszard Marciniak, Andrzej Szkaradkiewicz, Agnieszka Wasilewska, Izabela Chudzicka-Strugała, Hanna Ziuziakowska, Małgorzata Żebrowska, Przemysław Majewski, Tomasz Banasiewicz, Michał Drews
DOI: 10.5603/FHC.2011.0049
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Folia Histochem Cytobiol 2011;49(2):357-362.

open access

Vol 49, No 2 (2011)
Short Communication
Submitted: 2011-12-19
Published online: 2011-07-11

Abstract

In the present paper we correlate clinical data, as well as histopathological, immunohistochemical and molecular biology methods, with the occurrence of both forms of inflammatory bowel disease (IBD) i.e. ulcerative colitis and Crohn’s disease. We found that patients with a history of Epstein-Barr virus (EBV) or cytomegalovirus (CMV) infections, as well as steroid treatment, had increased susceptibility to the development of IBD. The diagnosis of IBD was confirmed by histopathology. Previous infections by EBV and CMV, as well as M. tuberculosis, were proved by PCR-based techniques and in situ hybridization. We found PCR-proved latent viral infections in 30–50% of the IBD patients we studied. However, we were unable to prove the presence of viral antigens by immunohistochemistry for EBV or CMV. We found positive correlations between the presence of anti-CMV IgG, as well as PCR-positive results for M. tuberculosis with an ulcerative colitis diagnosis. Additionally, up to 80% of IBD patients used steroids, which was found to be correlated with a diagnosis of Crohn’s disease. Our data may support the theory that IBD could be related to previous viral infections and the use of steroids. (Folia Histochemica et Cytobiologica 2011, Vol. 49, No. 2, 357–362)

Abstract

In the present paper we correlate clinical data, as well as histopathological, immunohistochemical and molecular biology methods, with the occurrence of both forms of inflammatory bowel disease (IBD) i.e. ulcerative colitis and Crohn’s disease. We found that patients with a history of Epstein-Barr virus (EBV) or cytomegalovirus (CMV) infections, as well as steroid treatment, had increased susceptibility to the development of IBD. The diagnosis of IBD was confirmed by histopathology. Previous infections by EBV and CMV, as well as M. tuberculosis, were proved by PCR-based techniques and in situ hybridization. We found PCR-proved latent viral infections in 30–50% of the IBD patients we studied. However, we were unable to prove the presence of viral antigens by immunohistochemistry for EBV or CMV. We found positive correlations between the presence of anti-CMV IgG, as well as PCR-positive results for M. tuberculosis with an ulcerative colitis diagnosis. Additionally, up to 80% of IBD patients used steroids, which was found to be correlated with a diagnosis of Crohn’s disease. Our data may support the theory that IBD could be related to previous viral infections and the use of steroids. (Folia Histochemica et Cytobiologica 2011, Vol. 49, No. 2, 357–362)

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Keywords

inflammatory bowel disease; histopathology; EBV; CMV

About this article
Title

Inflammatory bowel disease — is there something new in the immunological background?

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 2 (2011)

Article type

Brief communication

Pages

357-362

Published online

2011-07-11

Page views

2007

Article views/downloads

1848

DOI

10.5603/FHC.2011.0049

Bibliographic record

Folia Histochem Cytobiol 2011;49(2):357-362.

Keywords

inflammatory bowel disease
histopathology
EBV
CMV

Authors

Andrzej Marszałek
Ryszard Marciniak
Andrzej Szkaradkiewicz
Agnieszka Wasilewska
Izabela Chudzicka-Strugała
Hanna Ziuziakowska
Małgorzata Żebrowska
Przemysław Majewski
Tomasz Banasiewicz
Michał Drews

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