Vol 49, No 3 (2011)
Original paper
Submitted: 2012-01-05
Published online: 2011-10-28
Decreased serum level of IL-12 in the course of ischemia and reperfusion during abdominal aortic surgery
Monika Jedynak, Barbara Mroczko, Andrzej Siemiatkowski, Marek Gacko, Maciej Szmitkowski
DOI: 10.5603/FHC.2011.0066
·
Folia Histochem Cytobiol 2011;49(3):465-471.
Vol 49, No 3 (2011)
ORIGINAL PAPERS
Submitted: 2012-01-05
Published online: 2011-10-28
Abstract
Ischemic-reperfusion injury (IRI) is defined as tissue damage, organ dysfunction or failure developed
in the course of inflammatory response following ischemia and reperfusion (IR). Abdominal aortic aneurysm
(AAA) repair required IR of distal parts of the body carries a risk of organ injury and postoperative mortality of
between 4% and 12%. The aim of this study was the evaluation of IL-12 serum level during AAA repair in relation
to IR. Blood samples were taken before surgery (Preop), before aortic unclamping (Pre-Xoff), 90 min after unclamping
(90 min-Xoff) and 24 h after surgery (Postop) from 37 AAA patients; and before surgery (Preop), at 90 min of
surgery (90 min-surg), at 180 min of surgery (180 min-surg) and 24 h after operation (stop) from ten patients
scheduled for elective surgery of lumbar discopathy (SC); and once from ten healthy controls. IL-12 was measured
using the ELISA technique. Preoperative IL-12 was higher in AAA (0.21 pg/ml) and SC (0.31 pg/ml) patients than
in controls (0.05 pg/ml). A significant decrease in IL-12 (0.09 pg/ml) was observed at 90 min-Xoff in comparison to
the preoperative value in AAA but not in the SC group. 24 h after surgery, IL-12 levels were still low in the AAA
group (0.13 pg/ml), and nonsignificantly surpassed the preoperative value in the SC group (0.36 pg/ml). We conclude
that operative injury was associated with increased IL-12 levels, and IR with decreased IL-12 levels. Diminished
IL-12 during AAA repair might be associated with a higher risk of postoperative complications, but this
needs further evaluation. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 465–471)
Abstract
Ischemic-reperfusion injury (IRI) is defined as tissue damage, organ dysfunction or failure developed
in the course of inflammatory response following ischemia and reperfusion (IR). Abdominal aortic aneurysm
(AAA) repair required IR of distal parts of the body carries a risk of organ injury and postoperative mortality of
between 4% and 12%. The aim of this study was the evaluation of IL-12 serum level during AAA repair in relation
to IR. Blood samples were taken before surgery (Preop), before aortic unclamping (Pre-Xoff), 90 min after unclamping
(90 min-Xoff) and 24 h after surgery (Postop) from 37 AAA patients; and before surgery (Preop), at 90 min of
surgery (90 min-surg), at 180 min of surgery (180 min-surg) and 24 h after operation (stop) from ten patients
scheduled for elective surgery of lumbar discopathy (SC); and once from ten healthy controls. IL-12 was measured
using the ELISA technique. Preoperative IL-12 was higher in AAA (0.21 pg/ml) and SC (0.31 pg/ml) patients than
in controls (0.05 pg/ml). A significant decrease in IL-12 (0.09 pg/ml) was observed at 90 min-Xoff in comparison to
the preoperative value in AAA but not in the SC group. 24 h after surgery, IL-12 levels were still low in the AAA
group (0.13 pg/ml), and nonsignificantly surpassed the preoperative value in the SC group (0.36 pg/ml). We conclude
that operative injury was associated with increased IL-12 levels, and IR with decreased IL-12 levels. Diminished
IL-12 during AAA repair might be associated with a higher risk of postoperative complications, but this
needs further evaluation. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 465–471)
Keywords
surgery; interleukin-12; ischemia; reperfusion; abdominal aortic aneurysm
Title
Decreased serum level of IL-12 in the course of ischemia and reperfusion during abdominal aortic surgery
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 49, No 3 (2011)
Article type
Original paper
Pages
465-471
Published online
2011-10-28
Page views
1468
Article views/downloads
1538
DOI
10.5603/FHC.2011.0066
Bibliographic record
Folia Histochem Cytobiol 2011;49(3):465-471.
Keywords
surgery
interleukin-12
ischemia
reperfusion
abdominal aortic aneurysm
Authors
Monika Jedynak
Barbara Mroczko
Andrzej Siemiatkowski
Marek Gacko
Maciej Szmitkowski