Vol 49, No 3 (2011)
Original paper
Submitted: 2012-01-05
Published online: 2011-10-28
Different distribution of c-kit positive interstitial cells of Cajal-like in children’s urinary bladders
Anna Małgorzata Piaseczna-Piotrowska, Monika Dzieniecka, Andrzej Kulig, Marian Danilewicz, Andrzej Chilarski
DOI: 10.5603/FHC.2011.0061
·
Folia Histochem Cytobiol 2011;49(3):431-435.
Vol 49, No 3 (2011)
ORIGINAL PAPERS
Submitted: 2012-01-05
Published online: 2011-10-28
Abstract
We describe the presence of c-kit positive interstitial cells of Cajal-like (ICCs-like) in the walls of the
urinary bladders of children. An immunohistochemical study of specimens, obtained at autopsy from either the
trigonum (Group A) or the corpus (Group B), was performed using antibodies against c-kit (CD 117). Histological
morphometry of the immunoexpression of c-kit positive ICCs-like was performed by means of image analysis
system. The c-kit positive ICCs-like were identified by their morphology and counted in the vesical muscle
layer in ten adjacent high power fields, each of 0.0479 mm2. The areas of the epithelial and subepithelial layers
containing c-kit positive mast cells (rounded body with no dendritic processes) were neglected. The results were
expressed as the number of ICCs-like cells per mm2. Differences between groups were tested using unpaired
Student’s t-test preceded by evaluation of normality and Levene’s test. Results were considered statistically
significant if p < 0.05. In Group A, the mean number of ICCs-like cells was statistically significantly higher (41.5
cells/mm2) than in Group B (30.4 cells/mm2), p < 0.05. ICCs-like cells were found within the smooth muscle
layer of the urinary bladder. There was a different distribution of these cells in particular parts of the bladder,
which was probably due to the different roles of the trigonum and the corpus in the bladders of children. (Folia
Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 431–435)
Abstract
We describe the presence of c-kit positive interstitial cells of Cajal-like (ICCs-like) in the walls of the
urinary bladders of children. An immunohistochemical study of specimens, obtained at autopsy from either the
trigonum (Group A) or the corpus (Group B), was performed using antibodies against c-kit (CD 117). Histological
morphometry of the immunoexpression of c-kit positive ICCs-like was performed by means of image analysis
system. The c-kit positive ICCs-like were identified by their morphology and counted in the vesical muscle
layer in ten adjacent high power fields, each of 0.0479 mm2. The areas of the epithelial and subepithelial layers
containing c-kit positive mast cells (rounded body with no dendritic processes) were neglected. The results were
expressed as the number of ICCs-like cells per mm2. Differences between groups were tested using unpaired
Student’s t-test preceded by evaluation of normality and Levene’s test. Results were considered statistically
significant if p < 0.05. In Group A, the mean number of ICCs-like cells was statistically significantly higher (41.5
cells/mm2) than in Group B (30.4 cells/mm2), p < 0.05. ICCs-like cells were found within the smooth muscle
layer of the urinary bladder. There was a different distribution of these cells in particular parts of the bladder,
which was probably due to the different roles of the trigonum and the corpus in the bladders of children. (Folia
Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 431–435)
Keywords
interstitial cells of Cajal; CD 117; immunohistochemistry; urinary bladder
Title
Different distribution of c-kit positive interstitial cells of Cajal-like in children’s urinary bladders
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 49, No 3 (2011)
Article type
Original paper
Pages
431-435
Published online
2011-10-28
Page views
1644
Article views/downloads
1574
DOI
10.5603/FHC.2011.0061
Bibliographic record
Folia Histochem Cytobiol 2011;49(3):431-435.
Keywords
interstitial cells of Cajal
CD 117
immunohistochemistry
urinary bladder
Authors
Anna Małgorzata Piaseczna-Piotrowska
Monika Dzieniecka
Andrzej Kulig
Marian Danilewicz
Andrzej Chilarski