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Vol 43, No 3 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2011-08-22
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Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas

Włodzimierz Łuczyński, Oksana Kovalchuk, Maryna Krawczuk-Rybak, Małgorzata Mitura-Lesiuk, Iwona Malinowska, Lech Chyczewski, Jerzy Kowalczyk, Michał Matysiak
Folia Histochem Cytobiol 2005;43(3):169-171.

open access

Vol 43, No 3 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-08-22

Abstract

The complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th1/Th2/Th3/Tr1 balance is an interesting approach to explain immunological disturbances in lymphomas. The aim of our study was to assess mRNA for pro- and anti-inflammatory cytokines in T-cells in 20 children with Hodgkin- and non-Hodgkin lymphomas. CD4+ and CD8+ cells were isolated from whole peripheral blood and four different cytokine mRNA levels (IFN-γ, IL-10, IL-4, TGF-β) were determined by real-time PCR technique. Comparing to the control group, we found lower expression of mRNA for IFN-gamma in CD4+ cells at the time of lymphoma diagnosis. It may be one of the pathogenetic mechanisms of impaired immunity in these patients.

Abstract

The complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th1/Th2/Th3/Tr1 balance is an interesting approach to explain immunological disturbances in lymphomas. The aim of our study was to assess mRNA for pro- and anti-inflammatory cytokines in T-cells in 20 children with Hodgkin- and non-Hodgkin lymphomas. CD4+ and CD8+ cells were isolated from whole peripheral blood and four different cytokine mRNA levels (IFN-γ, IL-10, IL-4, TGF-β) were determined by real-time PCR technique. Comparing to the control group, we found lower expression of mRNA for IFN-gamma in CD4+ cells at the time of lymphoma diagnosis. It may be one of the pathogenetic mechanisms of impaired immunity in these patients.

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Keywords

Lymphoma; Children; T-cells; Interferon-gamma; Immunosuppression

About this article
Title

Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 3 (2005)

Article type

Original paper

Pages

169-171

Published online

2011-08-22

Page views

1054

Article views/downloads

937

Bibliographic record

Folia Histochem Cytobiol 2005;43(3):169-171.

Keywords

Lymphoma
Children
T-cells
Interferon-gamma
Immunosuppression

Authors

Włodzimierz Łuczyński
Oksana Kovalchuk
Maryna Krawczuk-Rybak
Małgorzata Mitura-Lesiuk
Iwona Malinowska
Lech Chyczewski
Jerzy Kowalczyk
Michał Matysiak

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