open access

Vol 43, No 3 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2011-08-22
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Quercetin suppresses heat shock-induced nuclear translocation of Hsp72

Joanna Jakubowicz-Gil, Bożena Pawlikowska-Pawlęga, Tomasz Piersiak, Jarosław Pawelec, Antoni Gawron
Folia Histochem Cytobiol 2005;43(3):123-128.

open access

Vol 43, No 3 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-08-22

Abstract

The effect of quercetin and heat shock on the Hsp72 level and distribution in HeLa cells was studied by Western blotting, indirect immunofluorescence and immunogold electron microscopy. In control cells and after quercetin treatment, Hsp72 was located both in the cytoplasm and in the nucleus in comparable amounts. After hyperthermia, the level of nuclear Hsp72 raised dramatically. Expression of Hsp72 in cytoplasm was also higher but not to such extent as that observed in the nucleus. Preincubation of heated cells with quercetin inhibited strong Hsp72 expression observed after hyperthermia and changed the intracellular Hsp72 distribution. The cytoplasmic level of protein exceeded the nuclear one, especially around the nucleus, where the coat of Hsp72 was noticed. Observations indicating that quercetin was present around and in the nuclear envelope suggested an involvement of this drug in the inhibition of nuclear translocation. Our results indicate that pro-apoptotic activity of quercetin may be correlated not only with the inhibition of Hsp72 expression but also with suppression of its migration to the nucleus.

Abstract

The effect of quercetin and heat shock on the Hsp72 level and distribution in HeLa cells was studied by Western blotting, indirect immunofluorescence and immunogold electron microscopy. In control cells and after quercetin treatment, Hsp72 was located both in the cytoplasm and in the nucleus in comparable amounts. After hyperthermia, the level of nuclear Hsp72 raised dramatically. Expression of Hsp72 in cytoplasm was also higher but not to such extent as that observed in the nucleus. Preincubation of heated cells with quercetin inhibited strong Hsp72 expression observed after hyperthermia and changed the intracellular Hsp72 distribution. The cytoplasmic level of protein exceeded the nuclear one, especially around the nucleus, where the coat of Hsp72 was noticed. Observations indicating that quercetin was present around and in the nuclear envelope suggested an involvement of this drug in the inhibition of nuclear translocation. Our results indicate that pro-apoptotic activity of quercetin may be correlated not only with the inhibition of Hsp72 expression but also with suppression of its migration to the nucleus.
Get Citation

Keywords

Quercetin - Hsp72 - Indirect immunofluorescence - Electron microscopy

About this article
Title

Quercetin suppresses heat shock-induced nuclear translocation of Hsp72

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 3 (2005)

Article type

Original paper

Pages

123-128

Published online

2011-08-22

Page views

1441

Article views/downloads

1050

Bibliographic record

Folia Histochem Cytobiol 2005;43(3):123-128.

Keywords

Quercetin - Hsp72 - Indirect immunofluorescence - Electron microscopy

Authors

Joanna Jakubowicz-Gil
Bożena Pawlikowska-Pawlęga
Tomasz Piersiak
Jarosław Pawelec
Antoni Gawron

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