open access
Vol 45, No 4 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2008-01-01
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Interchromatin granule clusters in vitellogenic oocytes of the flesh fly, Sarcophaga sp.
,
Folia Histochem Cytobiol 2007;45(4):401-403.
open access
Vol 45, No 4 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2008-01-01
Abstract
Insect oocyte nuclei contain different extrachromosomal nuclear bodies including Cajal bodies and interchromatin granule clusters (IGCs). In the present study, we describe IGC equivalents in the vitellogenic oocytes of the flesh fly, Sarcophaga sp. These structures were found to consist of 20-40-nm granules and also include the fibrillar areas of high and low electron density. Immunogold labeling electron microscopy revealed IGC marker protein SC35, Sm proteins, and trimethylguanosine cap of small nuclear (sn) RNAs in these bodies. Antibody against the non-phosphorylated RNA polymerase II selectively labeled the fibrillar areas of low electron density located inside the IGCs.
Abstract
Insect oocyte nuclei contain different extrachromosomal nuclear bodies including Cajal bodies and interchromatin granule clusters (IGCs). In the present study, we describe IGC equivalents in the vitellogenic oocytes of the flesh fly, Sarcophaga sp. These structures were found to consist of 20-40-nm granules and also include the fibrillar areas of high and low electron density. Immunogold labeling electron microscopy revealed IGC marker protein SC35, Sm proteins, and trimethylguanosine cap of small nuclear (sn) RNAs in these bodies. Antibody against the non-phosphorylated RNA polymerase II selectively labeled the fibrillar areas of low electron density located inside the IGCs.
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About this articleTitle
Interchromatin granule clusters in vitellogenic oocytes of the flesh fly, Sarcophaga sp.
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
401-403
Published online
2008-01-01
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1212
Bibliographic record
Folia Histochem Cytobiol 2007;45(4):401-403.
Authors
Dmitry Bogolyubov
Irina Stepanova
