Vol 46, No 4 (2008)
Original paper
Submitted: 2011-12-19
Published online: 2009-01-15
Expression of HLA-G in patients with B-cell chronic lymphocytic leukemia (B-CLL).
K Giannopoulos, A Dmoszyńska, A Bojarska-Junak, M Schmitt, J Roliński
DOI: 10.2478/v10042-008-0072-x
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Folia Histochem Cytobiol 2008;46(4):457-460.
Vol 46, No 4 (2008)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2009-01-15
Abstract
The expression of HLA-G was reported in certain malignancies and its role in escaping from immunosurveillance in cancers was proposed since HLA-G is a nonconventional HLA class I molecule that protects fetus from immunorecognition during pregnancy. Recent studies proposed HLA-G as novel prognostic marker for patients with B-CLL. HLA-G was showed to bear even better prognostic information compared to Zeta-chain associated protein of 70kDa (ZAP-70) and CD38 although some other authors did not find HLA-G expression in CLL. Therefore in this study we characterized the expression of HLA-G on both RNA and protein level. In most of 20 B-CLL patients we were able to detect signal from HLA-G using flow cytometry analysis. The expression of HLA-G was confirmed on messenger level by real-time RT-PCR experiments. No correlation between HLA-G expression and expression of well established prognostic factors such as ZAP-70 and CD38 was detected. These results confirm that HLA-G is expressed on CLL leukemic cells. Furthermore the expression of HLA-G on CLL cells suggests that this molecule might be involved in escaping of CLL cells from immunosurveillance.
Abstract
The expression of HLA-G was reported in certain malignancies and its role in escaping from immunosurveillance in cancers was proposed since HLA-G is a nonconventional HLA class I molecule that protects fetus from immunorecognition during pregnancy. Recent studies proposed HLA-G as novel prognostic marker for patients with B-CLL. HLA-G was showed to bear even better prognostic information compared to Zeta-chain associated protein of 70kDa (ZAP-70) and CD38 although some other authors did not find HLA-G expression in CLL. Therefore in this study we characterized the expression of HLA-G on both RNA and protein level. In most of 20 B-CLL patients we were able to detect signal from HLA-G using flow cytometry analysis. The expression of HLA-G was confirmed on messenger level by real-time RT-PCR experiments. No correlation between HLA-G expression and expression of well established prognostic factors such as ZAP-70 and CD38 was detected. These results confirm that HLA-G is expressed on CLL leukemic cells. Furthermore the expression of HLA-G on CLL cells suggests that this molecule might be involved in escaping of CLL cells from immunosurveillance.
Title
Expression of HLA-G in patients with B-cell chronic lymphocytic leukemia (B-CLL).
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 46, No 4 (2008)
Article type
Original paper
Pages
457-460
Published online
2009-01-15
Page views
2165
Article views/downloads
1794
DOI
10.2478/v10042-008-0072-x
Bibliographic record
Folia Histochem Cytobiol 2008;46(4):457-460.
Authors
K Giannopoulos
A Dmoszyńska
A Bojarska-Junak
M Schmitt
J Roliński
About this article