Vol 48, No 1 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2010-06-10
Expression of basal cell marker revealed by RAM11 antibody during epithelial regeneration in rabbits.
Grzegorz J Lis, Ewa Jasek, Jan A Litwin, Mariusz Gajda, Joanna Zarzecka, Tadeusz Cichocki
DOI: 10.2478/v10042-008-0087-3
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Folia Histochem Cytobiol 2010;48(1):89-92.
Vol 48, No 1 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2010-06-10
Abstract
RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular macrophages. Our previous report showed that RAM11 reacted with basal cells of stratified squamous epithelia of rabbit skin, oral mucosa and esophagus. The aim of the present study was to follow the appearance of RAM11 immunoreactivity in basal cells of regenerating oral epithelium in rabbits. No RAM11 immunostaining was observed in the regenerating epithelium examined on days 1 and 3 of wound healing. A weak immunofluorescence first appeared on day 7 in single basal cells and 32% of RAM11- positive basal cells were observed on day 14. These findings indicate that expression of the antigen recognized by RAM11 antibody is a transient event in the differentiation of oral keratinocytes which not always occurs during epithelial repair, although it is a constant feature of epithelial turnover in mature epithelium. Therefore this antigen can be regarded as basal cell marker only in mature stratified squamous epithelia.
Abstract
RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular macrophages. Our previous report showed that RAM11 reacted with basal cells of stratified squamous epithelia of rabbit skin, oral mucosa and esophagus. The aim of the present study was to follow the appearance of RAM11 immunoreactivity in basal cells of regenerating oral epithelium in rabbits. No RAM11 immunostaining was observed in the regenerating epithelium examined on days 1 and 3 of wound healing. A weak immunofluorescence first appeared on day 7 in single basal cells and 32% of RAM11- positive basal cells were observed on day 14. These findings indicate that expression of the antigen recognized by RAM11 antibody is a transient event in the differentiation of oral keratinocytes which not always occurs during epithelial repair, although it is a constant feature of epithelial turnover in mature epithelium. Therefore this antigen can be regarded as basal cell marker only in mature stratified squamous epithelia.
Title
Expression of basal cell marker revealed by RAM11 antibody during epithelial regeneration in rabbits.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 48, No 1 (2010)
Article type
Original paper
Pages
89-92
Published online
2010-06-10
Page views
2631
Article views/downloads
1803
DOI
10.2478/v10042-008-0087-3
Bibliographic record
Folia Histochem Cytobiol 2010;48(1):89-92.
Authors
Grzegorz J Lis
Ewa Jasek
Jan A Litwin
Mariusz Gajda
Joanna Zarzecka
Tadeusz Cichocki