Vol 48, No 1 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2010-06-10
Distributions of PCNA and Cas-3 in rat uterus during early pregnancy.
Hakan Oner, Jale Oner, Ramazan Demir
DOI: 10.2478/v10042-008-0088-2
·
Folia Histochem Cytobiol 2010;48(1):71-77.
Vol 48, No 1 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2010-06-10
Abstract
The aim of present study was to determine the distributions of proliferating cell nuclear agent (PCNA) and Cas-pase-3 (Cas-3) and their possible roles in implantation and decidualization during early pregnancy at immunohistochemical level. The tissue samples from pregnant animals between gestational days 1-5 were incubated by PCNA and Cas-3 antibodies and the obtained results were evaluated quantitatively. It was observed that PCNA immunoreactivity in uterine luminal epithelium and glandular epithelium reduced as from day 2 of gestation and disappeared as from day 4 of gestation. PCNA staining intensity in stromal cells and myometrium increased gradually with progressing gestation. While Cas-3 immunoreactivity was strongly detected in luminal and glandular epithelium throughout the whole gestational period, its reactivity markedly increased as from day 3 of gestation. In conclusion, it may suggest that the blastocyst implantation induces the uterine luminal epithelial cell death and stromal cell proliferation around the embryo in the uterus.
Abstract
The aim of present study was to determine the distributions of proliferating cell nuclear agent (PCNA) and Cas-pase-3 (Cas-3) and their possible roles in implantation and decidualization during early pregnancy at immunohistochemical level. The tissue samples from pregnant animals between gestational days 1-5 were incubated by PCNA and Cas-3 antibodies and the obtained results were evaluated quantitatively. It was observed that PCNA immunoreactivity in uterine luminal epithelium and glandular epithelium reduced as from day 2 of gestation and disappeared as from day 4 of gestation. PCNA staining intensity in stromal cells and myometrium increased gradually with progressing gestation. While Cas-3 immunoreactivity was strongly detected in luminal and glandular epithelium throughout the whole gestational period, its reactivity markedly increased as from day 3 of gestation. In conclusion, it may suggest that the blastocyst implantation induces the uterine luminal epithelial cell death and stromal cell proliferation around the embryo in the uterus.
Title
Distributions of PCNA and Cas-3 in rat uterus during early pregnancy.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 48, No 1 (2010)
Article type
Original paper
Pages
71-77
Published online
2010-06-10
Page views
2104
Article views/downloads
2031
DOI
10.2478/v10042-008-0088-2
Bibliographic record
Folia Histochem Cytobiol 2010;48(1):71-77.
Authors
Hakan Oner
Jale Oner
Ramazan Demir