Vol 48, No 4 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2011-04-12
Influence of thrombophlebitis on TGF-β1 and its signaling pathway in the vein wall.
Radoslaw Kowalewski, Andrzej Malkowski, Marek Gacko, Krzysztof Sobolewski
DOI: 10.2478/v10042-010-0041-z
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Folia Histochem Cytobiol 2010;48(4):542-548.
Vol 48, No 4 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-04-12
Abstract
Extensive extracellular matrix remodeling of the vein wall is involved in varicose veins pathogenesis. This process is controlled by numerous factors, including peptide growth factors. The aim of the study was to evaluate influence of thrombophlebitis on TGF-β1 and its signaling pathway in the vein wall. TGF-β1 mRNAlevels, growth factor content and its expression were evaluated by RT-PCR, ELISA, and western blot methods, respectively, in the walls of normal veins, varicose veins and varicose veins complicated by thrombophlebitis. Western blot analysis was used to assess TGF-β receptor type II (TGF-β RII) and p-Smad2/3 protein expression in the investigated material. Unchanged mRNA levels of TGF-β1, decreased TGF-β1 content, as well as decreased expression of latent and active forms of TGF-β1 were found in varicose veins. Increased expression of TGF-β RII and p-Smad2/3 were found in varicose veins. Thrombophlebitis led to increased protein expression of the TGF-β1 active form and p-Smad2/3 in the vein wall compared to varicose veins. TGF-β1 may play a role in the disease pathogenesis because of increased expression and activation of its receptor in the wall of varicose veins. Thrombophlebitis accelerates activation of TGF-β1 and activity of its receptor in the varicose vein wall.
Abstract
Extensive extracellular matrix remodeling of the vein wall is involved in varicose veins pathogenesis. This process is controlled by numerous factors, including peptide growth factors. The aim of the study was to evaluate influence of thrombophlebitis on TGF-β1 and its signaling pathway in the vein wall. TGF-β1 mRNAlevels, growth factor content and its expression were evaluated by RT-PCR, ELISA, and western blot methods, respectively, in the walls of normal veins, varicose veins and varicose veins complicated by thrombophlebitis. Western blot analysis was used to assess TGF-β receptor type II (TGF-β RII) and p-Smad2/3 protein expression in the investigated material. Unchanged mRNA levels of TGF-β1, decreased TGF-β1 content, as well as decreased expression of latent and active forms of TGF-β1 were found in varicose veins. Increased expression of TGF-β RII and p-Smad2/3 were found in varicose veins. Thrombophlebitis led to increased protein expression of the TGF-β1 active form and p-Smad2/3 in the vein wall compared to varicose veins. TGF-β1 may play a role in the disease pathogenesis because of increased expression and activation of its receptor in the wall of varicose veins. Thrombophlebitis accelerates activation of TGF-β1 and activity of its receptor in the varicose vein wall.
Title
Influence of thrombophlebitis on TGF-β1 and its signaling pathway in the vein wall.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 48, No 4 (2010)
Article type
Original paper
Pages
542-548
Published online
2011-04-12
Page views
2383
Article views/downloads
1985
DOI
10.2478/v10042-010-0041-z
Bibliographic record
Folia Histochem Cytobiol 2010;48(4):542-548.
Authors
Radoslaw Kowalewski
Andrzej Malkowski
Marek Gacko
Krzysztof Sobolewski
About this article