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Vol 48, No 4 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2011-04-12
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A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

Werner GÜtz, Solvig Lenz, Christoph Reichert, Kai-Olaf Henkel, Volker Bienengrä ber, Laura Pernicka, Karsten K H Gundlach, Tomasz Gredes, Thomas Gerber, Tomasz Gedrange, Friedhelm Heinemann
DOI: 10.2478/v10042-010-0096-x
·
Folia Histochem Cytobiol 2010;48(4):589-596.

open access

Vol 48, No 4 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-04-12

Abstract

To test the probable osteoinductive properties of NanoBone, a new highly non-sintered porous nano-crystalline hydroxylapatite bone substitute embedded into a silica gel matrix, granules were implanted subcutaneously and intramuscularly into the back region of 18 mini pigs. After periods of 5 and 10 weeks as well as 4 and 8 months, implantation sites were investigated using histological and histomorphometric procedures. Signs of early osteogenesis could already be detected after 5 weeks. The later periods were characterized by increasing membranous osteogenesis in and around the granules leading to the formation of bone-like structures showing periosteal and tendon-like structures with bone marrow and focal chondrogenesis. Bone formation was better in the subcutaneous than in the intramuscular implantation sites. This ectopic osteogenesis is discussed with regard to the nanoporosity and microporosity of the material, physico-chemical interactions at its surface, the differentiation of osteoblasts, the role of angiogenesis and the probable involvement of growth factors. The results of this preliminary study indicate that this biomaterial has osteoinductive potential and induces the formation of bone structures, mainly in subcutaneous adipose tissue in the pig.

Abstract

To test the probable osteoinductive properties of NanoBone, a new highly non-sintered porous nano-crystalline hydroxylapatite bone substitute embedded into a silica gel matrix, granules were implanted subcutaneously and intramuscularly into the back region of 18 mini pigs. After periods of 5 and 10 weeks as well as 4 and 8 months, implantation sites were investigated using histological and histomorphometric procedures. Signs of early osteogenesis could already be detected after 5 weeks. The later periods were characterized by increasing membranous osteogenesis in and around the granules leading to the formation of bone-like structures showing periosteal and tendon-like structures with bone marrow and focal chondrogenesis. Bone formation was better in the subcutaneous than in the intramuscular implantation sites. This ectopic osteogenesis is discussed with regard to the nanoporosity and microporosity of the material, physico-chemical interactions at its surface, the differentiation of osteoblasts, the role of angiogenesis and the probable involvement of growth factors. The results of this preliminary study indicate that this biomaterial has osteoinductive potential and induces the formation of bone structures, mainly in subcutaneous adipose tissue in the pig.

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About this article
Title

A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 48, No 4 (2010)

Article type

Original paper

Pages

589-596

Published online

2011-04-12

Page views

3581

Article views/downloads

3138

DOI

10.2478/v10042-010-0096-x

Bibliographic record

Folia Histochem Cytobiol 2010;48(4):589-596.

Authors

Werner GĂśtz
Solvig Lenz
Christoph Reichert
Kai-Olaf Henkel
Volker Bienengrä ber
Laura Pernicka
Karsten K H Gundlach
Tomasz Gredes
Thomas Gerber
Tomasz Gedrange
Friedhelm Heinemann

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