Identification of dendritic cells in the blood and synovial fluid of children with Juvenile Idiopathic Arthritis

Jacek Tabarkiewicz; Jacek Postępski; Edyta Olesińska; Jacek Roliński; Ewa Tuszkiewicz-Misztal

doi:10.5603/FHC.2011.0026

Vol 49, No 1 (2011)

Original paper

Submitted: 2011-12-19

Published online: 2011-04-19

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Identification of dendritic cells in the blood and synovial fluid of children with Juvenile Idiopathic Arthritis

Jacek Tabarkiewicz, Jacek Postępski, Edyta Olesińska, Jacek Roliński, Ewa Tuszkiewicz-Misztal

DOI: 10.5603/FHC.2011.0026

Folia Histochem Cytobiol 2011;49(1):188-199.

Vol 49, No 1 (2011)

ORIGINAL PAPERS

Submitted: 2011-12-19

Published online: 2011-04-19

Abstract

Childhood chronic arthritis of unknown etiology is known collectively as juvenile idiopathic arthritis (JIA) and consists of heterogeneous subtypes with unique clinical patterns of disease. JIA is the commonest rheumatic disease in children and may still result in significant disability, with joint deformity, growth impairment, and persistence of active arthritis into adulthood. Basic research is rather focused on rheumatoid arthritis, and this lead to small number of publications considering JIA. In this study we examine, by flow cytometry, the expression of dendritic cells (DCs) in the peripheral blood and synovial fluid of children with active JIA in a group of 220 patients. We reveal a significant decrease in the percentage of immature DCs in the blood of patients compared to control children. Surprisingly, we found higher percentages of mature circulating dendritic cells. Both populations of DCs, immature and mature, were accumulated in patients’ synovial fluid. We also confirmed the presence of CD206+/CD209+ in JIA samples, which can represent a population of macrophages with dendritic cells morphology. Our results support the thesis that dendritic cells are crucial in the induction and maintenance of autoimmune response and local inflammation during juvenile idiopathic arthritis. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 188–199)

Abstract

Childhood chronic arthritis of unknown etiology is known collectively as juvenile idiopathic arthritis (JIA) and consists of heterogeneous subtypes with unique clinical patterns of disease. JIA is the commonest rheumatic disease in children and may still result in significant disability, with joint deformity, growth impairment, and persistence of active arthritis into adulthood. Basic research is rather focused on rheumatoid arthritis, and this lead to small number of publications considering JIA. In this study we examine, by flow cytometry, the expression of dendritic cells (DCs) in the peripheral blood and synovial fluid of children with active JIA in a group of 220 patients. We reveal a significant decrease in the percentage of immature DCs in the blood of patients compared to control children. Surprisingly, we found higher percentages of mature circulating dendritic cells. Both populations of DCs, immature and mature, were accumulated in patients’ synovial fluid. We also confirmed the presence of CD206+/CD209+ in JIA samples, which can represent a population of macrophages with dendritic cells morphology. Our results support the thesis that dendritic cells are crucial in the induction and maintenance of autoimmune response and local inflammation during juvenile idiopathic arthritis. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 188–199)

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Keywords

dendritic cells; CD1c; CD303; CD83; CD206; CD209; arthritis; JIA

About this article

Title

Identification of dendritic cells in the blood and synovial fluid of children with Juvenile Idiopathic Arthritis

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 1 (2011)

Article type

Original paper

Pages

188-199

Published online

2011-04-19

Page views

1773

Article views/downloads

2293

DOI

10.5603/FHC.2011.0026

Bibliographic record

Folia Histochem Cytobiol 2011;49(1):188-199.

Keywords

dendritic cells
CD1c
CD303
CD83
CD206
CD209
arthritis
JIA

Authors

Jacek Tabarkiewicz
Jacek Postępski
Edyta Olesińska
Jacek Roliński
Ewa Tuszkiewicz-Misztal