Vol 50, No 1 (2012)
Original paper
Submitted: 2012-04-24
Published online: 2012-04-25
Evaluation of CD40, its ligand CD40L and Bcl-2 in psoriatic patients
Hanna Myśliwiec, Iwona Flisiak, Anna Baran, Maria Górska, Bożena Chodynicka
DOI: 10.5603/FHC.2012.0010
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Folia Histochem Cytobiol 2012;50(1):75-79.
Vol 50, No 1 (2012)
ORIGINAL PAPERS
Submitted: 2012-04-24
Published online: 2012-04-25
Abstract
Psoriasis is a chronic, recurrent, inflammatory disease. Recent investigations indicate an autoimmune pathogenesis of the disease. Apoptosis plays an important role in the regulation of immune mechanisms in many autoimmune diseases. Although CD40, CD40L, and Bcl-2 have already been studied in psoriatic skin lesions, little is known about their circulating forms. The aim of the present study was to evaluate the serum concentrations of Bcl-2, soluble CD40 and CD40L in psoriatic patients. The study was performed using ELISA kits in 39 psoriatic patients before treatment and after two weeks of topical ointment. Data was analyzed with respect to severity of psoriasis, duration of the disease, and coexisting psoriatic arthritis. Our results revealed that serum concentrations of soluble CD40 and CD40L before and after treatment were significantly higher (p < 0.01 and p < 0.001) in patients with psoriasis compared to the control group. Topical treatment of psoriatic lesions with dithranol ointment failed to decrease serum of CD40 and CD40L, which has not been described until now. There was no significant difference in serum Bcl-2 concentration between the compared groups. We did not find significant differences in serum concentrations of Bcl-2, CD40 or CD40L between patients with mild or severe psoriasis, nor any correlation between disease duration and the presence of psoriatic arthritis symptoms. Our data indicates upregulation of the CD40/CD40L system in psoriatic patients despite topical treatment and suggests their possible role in the pathogenesis of psoriasis.
Abstract
Psoriasis is a chronic, recurrent, inflammatory disease. Recent investigations indicate an autoimmune pathogenesis of the disease. Apoptosis plays an important role in the regulation of immune mechanisms in many autoimmune diseases. Although CD40, CD40L, and Bcl-2 have already been studied in psoriatic skin lesions, little is known about their circulating forms. The aim of the present study was to evaluate the serum concentrations of Bcl-2, soluble CD40 and CD40L in psoriatic patients. The study was performed using ELISA kits in 39 psoriatic patients before treatment and after two weeks of topical ointment. Data was analyzed with respect to severity of psoriasis, duration of the disease, and coexisting psoriatic arthritis. Our results revealed that serum concentrations of soluble CD40 and CD40L before and after treatment were significantly higher (p < 0.01 and p < 0.001) in patients with psoriasis compared to the control group. Topical treatment of psoriatic lesions with dithranol ointment failed to decrease serum of CD40 and CD40L, which has not been described until now. There was no significant difference in serum Bcl-2 concentration between the compared groups. We did not find significant differences in serum concentrations of Bcl-2, CD40 or CD40L between patients with mild or severe psoriasis, nor any correlation between disease duration and the presence of psoriatic arthritis symptoms. Our data indicates upregulation of the CD40/CD40L system in psoriatic patients despite topical treatment and suggests their possible role in the pathogenesis of psoriasis.
Keywords
psoriasis; bcl-2; CD40; CD40L
Title
Evaluation of CD40, its ligand CD40L and Bcl-2 in psoriatic patients
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 50, No 1 (2012)
Article type
Original paper
Pages
75-79
Published online
2012-04-25
Page views
1816
Article views/downloads
2346
DOI
10.5603/FHC.2012.0010
Bibliographic record
Folia Histochem Cytobiol 2012;50(1):75-79.
Keywords
psoriasis
bcl-2
CD40
CD40L
Authors
Hanna Myśliwiec
Iwona Flisiak
Anna Baran
Maria Górska
Bożena Chodynicka
About this article