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Vol 50, No 2 (2012)
Original paper
Submitted: 2012-01-19
Accepted: 2012-01-19
Published online: 2012-07-05
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Expression of cyclins A and E in melanocytic skin lesions and its correlation with some clinicopathologic features

Ana Alekseenko, Anna Wojas-Pelc, Grzegorz J. Lis, Zdzisław Wiśniowski, Magdalena Czerwińska, Łukasz Niewiara, Jan A. Litwin
DOI: 10.5603/FHC.2012.0035
·
Folia Histochem Cytobiol 2012;50(2):263-269.

open access

Vol 50, No 2 (2012)
ORIGINAL PAPERS
Submitted: 2012-01-19
Accepted: 2012-01-19
Published online: 2012-07-05

Abstract

Cyclins play a fundamental role in the cell cycle. Recent studies have focused on their role in the development of various malignancies. The objective of this study was to evaluate and compare the expression of cyclins A and E in common nevi, dysplastic nevi and malignant melanomas, and to investigate the relationship between cyclin expression and some pathological parameters such as tumor thickness, ulceration, regression, and mitotic rate, as well as several clinical and phenotypic parameters such as skin phototype, hair and eye color, number of nevi, personal or family melanoma history, and personal history of nonmelanoma skin cancer (NMSC). A total of 102 melanocytic skin lesions, including 30 common nevi, 38 dysplastic nevi and 34 melanomas, were examined. Expression of cyclins was detected by immunohistochemistry and quantified as a percentage of immunostained cell nuclei in each sample. Significant differences in expression of both cyclins were found between all lesion types: the median percentage of cyclin A-positive nuclei was 8.2% in melanomas, 3.4% in dysplastic nevi, and 0.95% in common nevi (p < 0.001). The corresponding percentages for cyclin E were 9.5%, 4.25% and 1.44% (p < 0.001). Expression of both cyclins was significantly higher among patients with a personal history of NMSC. Cyclin A was also significantly overexpressed in patients with a high total nevus count (TNC) compared to moderate and low TNC. Expression of cyclins did not significantly correlate with the other clinicopathologic features investigated. These findings indicate the possible involvement of cyclins A and E in the pathogenesis of malignant melanoma. Our results also show a potential diagnostic significance of these cyclins as markers allowing discrimination between dysplastic nevi and melanoma.

Abstract

Cyclins play a fundamental role in the cell cycle. Recent studies have focused on their role in the development of various malignancies. The objective of this study was to evaluate and compare the expression of cyclins A and E in common nevi, dysplastic nevi and malignant melanomas, and to investigate the relationship between cyclin expression and some pathological parameters such as tumor thickness, ulceration, regression, and mitotic rate, as well as several clinical and phenotypic parameters such as skin phototype, hair and eye color, number of nevi, personal or family melanoma history, and personal history of nonmelanoma skin cancer (NMSC). A total of 102 melanocytic skin lesions, including 30 common nevi, 38 dysplastic nevi and 34 melanomas, were examined. Expression of cyclins was detected by immunohistochemistry and quantified as a percentage of immunostained cell nuclei in each sample. Significant differences in expression of both cyclins were found between all lesion types: the median percentage of cyclin A-positive nuclei was 8.2% in melanomas, 3.4% in dysplastic nevi, and 0.95% in common nevi (p < 0.001). The corresponding percentages for cyclin E were 9.5%, 4.25% and 1.44% (p < 0.001). Expression of both cyclins was significantly higher among patients with a personal history of NMSC. Cyclin A was also significantly overexpressed in patients with a high total nevus count (TNC) compared to moderate and low TNC. Expression of cyclins did not significantly correlate with the other clinicopathologic features investigated. These findings indicate the possible involvement of cyclins A and E in the pathogenesis of malignant melanoma. Our results also show a potential diagnostic significance of these cyclins as markers allowing discrimination between dysplastic nevi and melanoma.

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Keywords

Cell cycle; Cyclin A; Cyclin E; Melanoma; Nevus

About this article
Title

Expression of cyclins A and E in melanocytic skin lesions and its correlation with some clinicopathologic features

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 2 (2012)

Article type

Original paper

Pages

263-269

Published online

2012-07-05

Page views

2034

Article views/downloads

1953

DOI

10.5603/FHC.2012.0035

Bibliographic record

Folia Histochem Cytobiol 2012;50(2):263-269.

Keywords

Cell cycle
Cyclin A
Cyclin E
Melanoma
Nevus

Authors

Ana Alekseenko
Anna Wojas-Pelc
Grzegorz J. Lis
Zdzisław Wiśniowski
Magdalena Czerwińska
Łukasz Niewiara
Jan A. Litwin

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